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FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2465..2561) /label=attL2 /note="attL2 (c)" misc_signal complement(2576..2595) /note="T7 Promoter/priming site (c)" primer_bind 2603..2619 /note="M13 Reverse priming site" gene 2732..3541 /note="Kanamycin resistance gene" rep_origin 3662..4335 /note="pUC origin" vector join(2473..4338,1..651) /source="pDONR%99221" /type="Donor Vector" CDS 671..2458 /label=ORF1 misc_feature 671..691 /note="TEV site" misc_feature 692..2458 /note="optimized GLCE coding region" source 1..4338 /dnas_title="GLCE-pDONR" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTCT GAGAACCTGT ATTTTCAAGG CAACAAGTGC AGCAGCGACA AGGCCATCCA 721 GTTCCCCAGA AGAAGCAGCA GCGGCTTCCG GGTGGACGGC TTCGAGAAAA GAGCCGCCGC 781 TAGCGAGAGC AACAACTACA TGAACCACGT GGCCAAGCAG CAGAGCGAGG AAGCCTTCCC 841 ACAGGAACAG CAGAAAGCCC CCCCTGTCGT GGGCGGCTTC AACAGCAATG TGGGCAGCAA 901 GGTGCTGGGC CTGAAGTACG AGGAAATCGA CTGCCTGATC AACGACGAGC ACACCATCAA 961 GGGCAGACGC GAGGGCAACG AGGTGTTCCT GCCTTTCACC TGGGTGGAAA AGTACTTCGA 1021 CGTGTACGGC AAGGTGGTGC AGTACGACGG CTACGACAGA TTCGAGTTCA GCCACAGCTA 1081 CTCCAAGGTG TACGCCCAGA GAGCCCCCTA CCACCCCGAT GGCGTGTTCA TGAGCTTCGA 1141 GGGCTACAAC GTGGAAGTGC GGGACAGAGT GAAGTGCATC AGCGGCGTGG AAGGCGTGCC 1201 ACTGAGCACA CAGTGGGGCC CTCAGGGCTA CTTCTACCCC ATCCAGATCG CCCAGTACGG 1261 CCTGAGCCAC TACAGCAAGA ACCTGACCGA GAAGCCCCCT CACATCGAGG TGTACGAGAC 1321 AGCCGAGGAC CGGGACAAGA ACAAGCCCAA CGACTGGACC GTGCCCAAGG GCTGCTTCAT 1381 GGCCAACGTG GCCGACAAGA GCCGGTTCAC CAACGTGAAG CAGTTTATCG CCCCCGAGAC 1441 AAGCGAGGGC GTGTCCCTGC AGCTGGGCAA CACCAAGGAC TTCATCATCA GCTTCGACCT 1501 GAAGTTCCTG ACCAACGGCA GCGTGTCCGT GGTGCTGGAA ACCACCGAGA AGAACCAGCT 1561 GTTCACCATC CACTACGTGT CCAACGCCCA GCTGATCGCC TTCAAAGAGC GGGACATCTA 1621 CTACGGCATC GGCCCCAGAA CCTCTTGGAG CACCGTGACC AGGGACCTCG TGACCGACCT 1681 GAGAAAGGGC GTGGGCCTGA GCAATACCAA GGCCGTGAAG CCCACCAAGA TCATGCCCAA 1741 GAAAGTCGTG CGCCTGATCG CCAAGGGCAA GGGCTTCCTG GACAACATCA CCATCAGCAC 1801 CACCGCCCAC ATGGCCGCTT TCTTCGCCGC CTCTGATTGG CTCGTGCGGA ACCAGGATGA 1861 GAAGGGCGGC TGGCCCATCA TGGTCACCAG AAAGCTGGGC GAGGGCTTCA AGAGCCTGGA 1921 ACCCGGCTGG TACTCTGCCA TGGCTCAGGG CCAGGCCATC TCTACACTCG TGCGGGCCTA 1981 CCTGCTGACC AAGGACCACA TCTTTCTGAA CAGCGCCCTG CGGGCTACCG CCCCCTACAA 2041 GTTTCTGTCT GAGCAGCACG GCGTGAAGGC CGTGTTTATG AACAAGCACG ATTGGTACGA 2101 AGAGTACCCC ACCACCCCCA GCAGCTTCGT GCTGAACGGC TTCATGTACA GCCTGATCGG 2161 CCTGTACGAC CTGAAAGAGA CTGCCGGCGA GAAGCTGGGA AAAGAGGCCC GGTCCCTGTA 2221 CGAGCGGGGC ATGGAATCTC TGAAGGCCAT GCTGCCTCTG TACGACACCG GCAGCGGCAC 2281 CATCTACGAC CTGCGGCACT TCATGCTGGG AATCGCCCCC AACCTGGCCA GATGGGACTA 2341 CCACACCACC CACATCAACC AGCTGCAGCT GCTGAGCACC ATCGACGAGA GCCCCGTGTT 2401 CAAAGAATTC GTGAAGCGGT GGAAGTCCTA CCTGAAGGGC AGCCGGGCCA AGCACAACTA 2461 GGACCCAGCT TTCTTGTACA AAGTTGGCAT TATAAGAAAG CATTGCTTAT CAATTTGTTG 2521 CAACGAACAG GTCACTATCA GTCAAAATAA AATCATTATT TGCCATCCAG CTGATATCCC 2581 CTATAGTGAG TCGTATTACA TGGTCATAGC TGTTTCCTGG CAGCTCTGGC CCGTGTCTCA 2641 AAATCTCTGA TGTTACATTG CACAAGATAA AATAATATCA TCATGAACAA TAAAACTGTC 2701 TGCTTACATA AACAGTAATA CAAGGGGTGT TATGAGCCAT ATTCAACGGG AAACGTCGAG 2761 GCCGCGATTA AATTCCAACA TGGATGCTGA TTTATATGGG TATAAATGGG CTCGCGATAA 2821 TGTCGGGCAA TCAGGTGCGA CAATCTATCG CTTGTATGGG AAGCCCGATG CGCCAGAGTT 2881 GTTTCTGAAA CATGGCAAAG GTAGCGTTGC CAATGATGTT ACAGATGAGA TGGTCAGACT 2941 AAACTGGCTG ACGGAATTTA TGCCTCTTCC GACCATCAAG CATTTTATCC GTACTCCTGA 3001 TGATGCATGG TTACTCACCA CTGCGATCCC CGGAAAAACA GCATTCCAGG TATTAGAAGA 3061 ATATCCTGAT TCAGGTGAAA ATATTGTTGA TGCGCTGGCA GTGTTCCTGC GCCGGTTGCA 3121 TTCGATTCCT GTTTGTAATT GTCCTTTTAA CAGCGATCGC GTATTTCGTC TCGCTCAGGC 3181 GCAATCACGA ATGAATAACG GTTTGGTTGA TGCGAGTGAT TTTGATGACG AGCGTAATGG 3241 CTGGCCTGTT GAACAAGTCT GGAAAGAAAT GCATAAACTT TTGCCATTCT CACCGGATTC 3301 AGTCGTCACT CATGGTGATT TCTCACTTGA TAACCTTATT TTTGACGAGG GGAAATTAAT 3361 AGGTTGTATT GATGTTGGAC GAGTCGGAAT CGCAGACCGA TACCAGGATC TTGCCATCCT 3421 ATGGAACTGC CTCGGTGAGT TTTCTCCTTC ATTACAGAAA CGGCTTTTTC AAAAATATGG 3481 TATTGATAAT CCTGATATGA ATAAATTGCA GTTTCATTTG ATGCTCGATG AGTTTTTCTA 3541 ATCAGAATTG GTTAATTGGT TGTAACACTG GCAGAGCATT ACGCTGACTT GACGGGACGG 3601 CGCAAGCTCA TGACCAAAAT CCCTTAACGT GAGTTACGCG TCGTTCCACT GAGCGTCAGA 3661 CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG 3721 CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC 3781 AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTTCTTCT 3841 AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC 3901 TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT 3961 GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG 4021 CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT 4081 ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG 4141 GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG 4201 TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG 4261 GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG 4321 GCCTTTTGCT CACATGTT //